A postdoctoral position is available in Dr. Shuo Gu’s lab in the RNA Biology Laboratory (RBL), Center for Cancer Research (CCR) – Frederick, MD, National Cancer Institute (NCI), National Institutes of Health (NIH). The candidate will perform basic research on small RNA functions by using a mouse model.
The goal of our program is to uncover the mechanisms by which the function of miRNA is regulated in health and disease and to apply those in developing effective treatments for cancer. Using genetic functional studies in living cells and animals, together with biochemical and next-generation sequencing (NGS) approaches, our laboratory focuses on elucidating mechanisms of miRNA biogenesis and the roles which miRNAs play in tumorigenesis.
In particular, we are interested in studying the biogenesis and function of miRNA isoforms (isomiRs). It is well-established that miRNAs play critical role in gene regulation and diseases such as cancer. The function of isomiRs, on the other hand, is less defined. Multiple lines of evidence suggest that isomiRs possess unique biological roles 1. We have developed a novel algorithm to accurately detect and analyze miRNA isomiRs based on next-generation-sequencing (NGS) data 2. We obtained insights into how 5’ isomiRs are generated 3 and uncovered a way in which 3’ tailing confers miRNAs with new specificities 4. Recently, we revealed one mechanism how 3’ tailing impacts miRNA stability 5. These findings identified mechanisms by which miRNAs diversify their functions via sequence modifications at both the 5’ and 3’ ends. We will extend our studies beyond transformed human cell lines to more physiological relevant mouse models. Combining classical biochemistry techniques with high-throughput sequencing, the successful candidate will continue revealing novel biological functions of isomiRs in the context of cancer, which will not only establish the biological relevance of our findings, but also may lead to translational applications.
Interested candidates must have a Ph.D. and/or an M.D. Students who just received or expect to receive their degree in this summer, are particularly encouraged to apply. Applicants must have a strong background in molecular biology and biochemistry. Experience with mouse study, RNA research and/or deep sequencing data analysis is a strong plus. Salary is commensurate with research experience and accomplishments. Applications are assessed on a rolling basis and interested applicants are encouraged to apply asap by sending a cover letter, CV and contact information for three references to firstname.lastname@example.org
Recent publications from our group:
1. IsomiRs: Expanding the miRNA repression toolbox beyond the seed. Biochim Biophys Acta Gene Regul Mech. 2019 Apr 4
2. QuagmiR: a cloud-based application for isomiR big data analytics. Bioinformatics. 2019 May
3. Differences between Pri-miRNA Paralogs Promote Alternative Drosha Cleavage and Expand Target Repertoires. Cell Rep. 2019 Jan 8;26(2):447-459.e4.
4. 3′ Uridylation Confers miRNAs with Non-canonical Target Repertoires. Mol Cell. 2019 Jun
5. AGO-bound Mature miRNAs are Oligouridylated by TUTs and Subsequently Degraded by DIS3L2. Nat Commun. 2020 Jun 2
This position is subject to a background investigation. The NIH is dedicated to building a diverse community in its training and employment programs; underrepresented minority candidates are encouraged to apply.
DHHS, NIH, and NCI are Equal Opportunity EmployersMehr
|Titel||Postdoctoral Position at NIH/NCI|
|Employer||National Institutes of Health|
|Job location||Frederick, Maryland, Frederick|
|Veröffentlicht||Juni 30, 2020|
|Jobart||Post Doc  |
|Fachbereiche||Krebsforschung,   Biochemie,   Bioinformatik,   Molekularbiologie,   Genetik,   Zellbiologie  |